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Elena V. Eremeeva
Laboratory of Photobiology
Institute of Biophysics
Akademgorodok 660036 Krasnoyarsk Russia
Tel: (3912) 494430
E-mail: l_eremeeva@mail.ru
EDUCATIONAL BACKGROUND
Ph.D. thesis Molecular mechanism of active photoprotein complex formation
2013 Ph.D., Biochemistry, Wageningen University, Wageningen, The Netherlands
2010 Ph.D., Biophysics, Institute of Biophysics SB RAS, Krasnoyarsk, Russia
2005 M.Sc., Biochemistry, Krasnoyarsk State University, Krasnoyarsk, Russia
PROFESSIONAL EXPERIENCE
2008-present Laboratory of Photobiology, Institute of Biophysics SB RAS, research scientists (2012-present), junior research scientist (2008-2012)
INTERNATIONAL PROFESSIONAL TRAVEL
2012-2013 (August-January) Collaborative research funded by Sandwich PhD fellowship, Wageningen University, Wageningen, The Netherlands
2011 (March-July) Collaborative research funded by Sandwich PhD fellowship, Wageningen University, Wageningen, The Netherlands
2010 (October-December) Collaborative research, the National Lab of Biomacromolecules, Institute of Biophysics, Beijing, China
2009 (April-June) Collaborative research, the National Lab of Biomacromolecules, Institute of Biophysics, Beijing, China
2007 (February-September) Collaborative research funded by Sandwich PhD fellowship, Wageningen University, Wageningen, The Netherlands
INTERNATIONAL CONFERENCES/SEMINARS ATTENDED
2016 19th Int. Symposium on Bioluminescence and Chemiluminescence, Tsukuba, Japan
2014 18th International Symposium on Flavins and Flavoproteins, Cha-Am, Thailand
2012 NWO Study Group Meeting Biomolecular Chemistry, Lunteren, The Netherlands
2012 17th Int. Symposium on Bioluminescence and Chemiluminescence, Guelph, Canada
2010 35th FEBS Congress, Gothenburg, Sweden
2008 15th Int. Symposium on Bioluminescence and Chemiluminescence, Shanghai, China, winner of Marlene DeLuca Prize for young scientists.
PUBLICATIONS
PAPERS IN PEER REVIEWD JOURNALS
Eremeeva E.V., Vysotski E.S. (2018) Exploring bioluminescence function of the Ca2+ -regulated photoproteins with site-directed mutagenesis. Photochem Photobiol., doi: 10.1111/php.12945.
Eremeeva E.V., Vysotski E.S. (2017) Bioluminescent and biochemical properties of Cys-free Ca2+-regulated photoproteins obelin and aequorin. J Photochem Photobiol B., 174, 97-105.
Eremeeva E.V., Bartsev S.I., van Berkel W.J., Vysotski E.S. (2017) Unanimous model for describing the fast bioluminescence kinetics of Ca2+-regulated photoproteins of different organisms. Photochem Photobiol., 93, 495-502.
Burakova L.P., Natashin P.V., Markova S.V., Eremeeva E.V., Malikova N.P., Cheng C., Liu Z.J., Vysotski E.S. (2016) Mitrocomin from the jellyfish Mitrocoma cellularia with deleted C-terminal tyrosine reveals a higher bioluminescence activity compared to wild type photoprotein. J Photochem Photobiol B., 162, 286-297.
Burakova L.P., Stepanyuk G.A., Eremeeva E.V., Vysotski E.S. (2016) Role of certain amino acid residues of the coelenterazine-binding cavity in bioluminescence of light-sensitive Ca(2+)-regulated photoprotein berovin. Photochem Photobiol Sci., 15, 691-704.
Eremeeva E.V., van Berkel W.J., Vysotski E.S. (2016) Transient-state kinetic analysis of complex formation between photoprotein clytin and GFP from jellyfish Clytia gregaria. FEBS Lett., 590, 307-316.
Natashin P.V., Ding W., Eremeeva E.V., Markova S.V., Lee J., Vysotski E.S., Liu Z.-J. (2014) Structures of the Ca2+-regulated photoprotein obelin Y138F mutant before and after bioluminescence support the catalytic function of a water molecule in the reaction. Acta Crystallogr. D Biol. Crystallogr., 70, 720–732
Eremeeva E.V., Burakova L.P., Krasitskaya V.V., Kudryavtsev A.N., Shimomura O., Frank L.A. (2014) Hydrogen-bond networks between the C-terminus and Arg from the first a-helix stabilize photoprotein molecules. Photochem. Photobiol. Sci., 13, 541-547.
Eremeeva E.V., Markova S.V., van Berkel W.J.H., Vysotski E.S. (2013) Role of key residues of obelin in coelenterazine binding and conversion into 2-hydroperoxy adduct. J. Photochem. Photobiol. B, 127, 133-139.
Eremeeva, E.V., Markova, S.V., Vysotski, E.S. (2013) Highly active BRET-reporter based on yellow mutant of Renilla muelleri luciferase. Dokl. Biochem. Biophys., 450, 147-150.
Eremeeva, E.V., Natashin, P.V., Song, L., Zhou, Y.G., van Berkel, W.J.H., Liu, Z.J, Vysotski, E.S. (2013) Oxygen activation of apo-obelin-coelenterazine complex. Chembiochem., 14, 739-745.
Eremeeva, E.V., Markova, S.V., Frank, L.A., Visser, A.J.W.G., van Berkel, W.J.H., Vysotski, E.S. (2013) Bioluminescent and spectroscopic properties of His-Trp-Tyr triad mutants of obelin and aequorin. Photoch. Photobio. Sci., 12, 1016-1024.
Eremeeva, E.V., Vysotski, E.S., Westphal, A.H., van Mierlo, C.P.M., van Berkel, W.J.H. (2012) Ligand binding and conformational states of the photoprotein obelin. FEBS Lett., 586, 4173–4179.
Eremeeva, E.V., Frank, L.A., Markova S.V., Vysotski, E.S. (2010) Ca2+-regulated photoprotein obelin as N-terminal partner in the fusion proteins. Journal of Siberian Federal University, B4, 372-383.
van Oort, B., Eremeeva, E.V., Koehorst, R.B.M., Laptenok, S.P., van Amerongen, H., van Berkel, W.J.H., Malikova, N.P., Markova, S.V., Vysotski, E.S., Visser, A.J.W.G., Lee, J. (2009) Picosecond fluorescence relaxation spectroscopy of the calcium-discharged photoproteins aequorin and obelin. Biochemistry, 48, 10486-10491.
Eremeeva, E.V., Markova, S.V., Westphal, A.H., Visser, A.J.W.G., van Berkel, W.J.H., Vysotski, E.S. (2009) The intrinsic fluorescence of apo-obelin and apo-aequorin and use of its quenching to characterize coelenterazine binding. FEBS Lett., 583, 1939-1944.
MONOGRAPH
Eremeeva E.V. Molecular mechanism of active photoprotein complex formation. Print Service Ede, The Netherlands, 2013, 195 pages. ISBN 978-94-6173-458-7.
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