Prof. VALENTINA A. KRATASYUK, Ph.D., D.Sc.

Professor, Head, Department of Biophysics, Siberian Federal University (former Krasnoyarsk state university), Institute of Fundamental Biology and Biotechnology

pr. Svobodnii, 79, aud. 13-08, Krasnoyarsk, Russia

Phone: +7(391)206-20-72, 2062307, 249-56-42, +7-967-608-56-43,

Fax: +7(391)20-62-166

E-mail: valkrat@mail.ru

Education

• Specialist, Biology (Biochemistry), Novosibirsk State University, School of Natural Science, Novosibirsk, Russia, 1975.

• Kandidatskaya degree (Ph.D.), Biological Sciences (Biophysics), Institute of Biophysics, Siberian Branch of Russian Academy of Sciences, Krasnoyarsk, Russia, 1985.

• Doctoral degree (D.Sc.), Biological Sciences (Biophysics), Institute of Biophysics, Siberian Branch of Russian Academy of Sciences, Krasnoyarsk, Russia, 1995.

Current Position

• Since 1999 - Professor, Head, Department of Biophysics, Institute of Fundamental Biology and Biotechnology, Siberian Federal University (former Krasnoyarsk state university), Krasnoyarsk

• Since 1995 - Leading Researcher, Laboratory of Photobiology, Institute of Biophysics, Siberian Branch of Russian Academy of Sciences, Krasnoyarsk

• Since 2007 - Courtesy Professor of Agricultural and Biological Engineering Department at the University of Florida (USA)

• Since 2006 - Director of Research, Applied BioSystems, Ltd, Krasnoyarsk

Previous Positions

• 1996 – 2003 – Professor, lecturer in Biology, Department of High School Pedagogy, Krasnoyarsk State University, Krasnoyarsk.

• 1989 – 1996 – Lecturer in Biology, Department of Physiology and Biochemistry, Krasnoyarsk State University, Krasnoyarsk.

• 1981 – 1994 – Researcher, post-doc, senior researcher, Laboratory of Photobiology, Institute of Biophysics, USSR Academy of Sciences, Siberian Branch, Krasnoyarsk.

• 1975 – 1981 – Laboratory assistant, junior researcher, Laboratory of Photobiology, L.V.Kirensky Institute of Physics, USSR Academy of Sciences, Siberian Branch, Krasnoyarsk.

• 1974 – 1975 – Laboratory assistant, Scientific Research Department, Novosibirsk State University, Novosibirsk.

Memberships

• The Scientific Council of the International Society of Bioluminescence and Chemiluminescence, the council.

• The member, Organizing Committee of 9, 10, 11, 12, 13, 14, 15 International Symposium on Bio- and Chemiluminescence

• The member of Council of the International Society of Environmental Toxicology and Chemistry.

• The member of Asian Federation of Biotechnology

• The member of Russian Fulbright Alumni

• The member of Russian Society of Biotechnologist

• The member of Russian National Committee of Biophysicist

• The member of Russian Society for Photobiology

• The member of International Society on Bioencapsulation.

• The member of Russian Biochemical Society.

• The member of the European Society for Photobiology.

Research Interests

• Mechanisms of bioluminescence, bioluminescent enzymatic biosensors, bioluminescent toxicity bioassays, bioluminescent analysis, biotechnological design, biochemistry, science education, education based on bioluminescence, history of sciences, immobilization of enzymes. bioluminescence, biotechnological design, biosensors, enzymatic toxicity bioassays, laboratory diagnostics, plant stress, storekeeping, ecological monitoring, luciferase, biotesting, soil, multicomponent immobilized reagent, coupled enzyme system

Research Projects

• Indication of redox processes by bioluminescent method. Development of bioluminescent assays to monitor the aquatic ecosystems marked by redox properties

• Bioluminescent Signal System (BSS) for early anti-terrorism application

• Molecular mechanisms of emitter’s formation in bioluminescent reactions of various luminous organisms

• Bioluminescent analysis: biosensors and biocatalitic technologies. Bioluminescent biosensors for toxicity analysis in biological systems

• Gel modelling of luciferase surrounding inside cell of marine luminous bacteria

• Enzyme-substrate interactions in immobilized multicomponent reagent for bioluminescent tests

• The reactive oxygen species in bacterial bioluminescence

• Experimental verification of the hypothesis on activity of upper electron excited states for different bioluminescent organisms

• Effect of micro environmental change on kinetic parameters of steady-state and non-steady-state enzyme-induced bioluminescent reactions

• The investigation of stress in plants by bioluminescent methods

Basic Results in Scientific Activities

• Development of technology commercialization of bioluminescent assays and biosensors using enzymes of marine luminous bacteria.

• Development of new bioluminescent enzymatic biosensors using luminous bacteria enzymes based on nanobiotechnology

• Development of new bioluminescent enzymatic bioassays using enzymes of marine and recombinant luminous bacteria for ecological monitoring of water in natural and artificial water ecosystems, air and water in closed ecosystems for ecology, Space biotechnology, medicine and biotechnology.

• Development of new bioluminescent assays for medicine.

• Development of immobilized multicomponent reagents for bioluminescent assays. The experimental model of in vivo bacterial bioluminescence based on immobilized bioluminescent enzymes.

• Bio-alarm Laboratory has been designed for bioluminescent monitoring of the “health” of water ecosystems and to provide early warnings about anomalies arising due to anthropogenic and natural factors. Taking part in a series of ecological expeditions to the salt lake Shira, river Yenisei, Japanese sea.

• Development and implementation of bioluminescent toxicity bioassays based on enzymatic reactions catalized by bacterial luciferases (from marine luminous bacteria). Designing of biophysical biosensors for xenobiotic assays in ecology, medicine and agriculture.

• Development of biophysical and biochemical mechanisms of inhibition of bacterial bioluminescence.

Publications: more 250: 88 Referred Journal Articles, 3 Book Coauthored, 2 Books Edited, 3 Book Chapters, 16 Patents, more 100 Abstracts, 43 Meeting Papers, more 30 Education Publications, 3 Education Software Packages Developed and Other Publications

Recent selected publications include:

1. Esimbekova E., Kratasyuk V., Shimomura O. Application of Enzyme Bioluminescence in Ecology //Bioluminescence: Fundamentals and Applications in Biotechnology-Volume 1. – Springer Berlin Heidelberg, Thouand G., Marks R. (eds.), 2014. – С. 67-109., 55illus., 24 illus. in color., Hardcover ISBN 978-3-662-43384-3

2. Bezrukikh A., Esimbekova E., Kratasyuk V. Gelatin and Starch Media Stabilize Bacterial Luciferase and Oxidoreductase //LUMINESCENCE. – 111 RIVER ST, HOBOKEN 07030-5774, NJ USA : WILEY-BLACKWELL, 2014. – Т. 29. – С. 73-74.

3. Deeva A. A., Nemtseva E. V., Kratasyuk V. A. Structural properties of tryptophan microenvironment in bacterial luciferase //LUMINESCENCE. – 111 RIVER ST, HOBOKEN 07030-5774, NJ USA : WILEY-BLACKWELL, 2014. – Т. 29. – С. 72-73.

4. Asanova A., Esimbekova E., Kratasyuk V. Bioluminescent Enzymatic Methods for Toxicological Safety Testing of Food Additives //LUMINESCENCE. – 111 RIVER ST, HOBOKEN 07030-5774, NJ USA : WILEY-BLACKWELL, 2014. – Т. 29. – С. 74-74.

5. Kratasyuk V., Esimbekova E. Application of enzyme bioluminescence in ecology //LUMINESCENCE. – 111 RIVER ST, HOBOKEN 07030-5774, NJ USA : WILEY-BLACKWELL, 2014. – Т. 29. – С. 25-25.

6. Lonshakova-Mukina V., Esimbekova E., Kratasyuk V. Biological module of bioluminescent biosensor for environmental toxicity assay //LUMINESCENCE. – 111 RIVER ST, HOBOKEN 07030-5774, NJ USA : WILEY-BLACKWELL, 2014. – Т. 29. – С. 72-72.

7. Sukovataya I., Kratasyuk V., Gitelson J. Bioluminescence as an educational tool //LUMINESCENCE. – 111 RIVER ST, HOBOKEN 07030-5774, NJ USA : WILEY-BLACKWELL, 2014. – Т. 29. – С. 46-46.

8. Lisitsa A. A., Nemtseva E. V., Kratasyuk V. A. Optical correction of the results of bioassays based on bacterial bioluminescence in vitro applied to nanoparticles //LUMINESCENCE. – 111 RIVER ST, HOBOKEN 07030-5774, NJ USA : WILEY-BLACKWELL, 2014. – Т. 29. – С. 74-75.

9. Sumarokova M., Sukovataya I., Kratasyuk V. Effect of pH on thermal stability of bioluminescent coupled enzyme system NAD (P) H: FMN-oxidoreductase-luciferase //LUMINESCENCE. – 111 RIVER ST, HOBOKEN 07030-5774, NJ USA : WILEY-BLACKWELL, 2014. – Т. 29. – С. 96-97.

10. Rimatskaia N., Sutormin O., Sukovataya I., Kratasyuk V. Bioluminescence in education: Contemporary bioluminescent practical training session for research activities in secondary schools //LUMINESCENCE. – 111 RIVER ST, HOBOKEN 07030-5774, NJ USA : WILEY-BLACKWELL, 2014. – Т. 29. – С. 75-76.

11. Sutormin O., Sukovataya I., Kratasyuk V. Effect of structured microenvironments on stability of coupled enzyme system ADH: FMN-oxidoreductase-luciferase //LUMINESCENCE. – 111 RIVER ST, HOBOKEN 07030-5774, NJ USA : WILEY-BLACKWELL, 2014. – Т. 29. – С. 97-98.

12. Rimatskaia N., Baigina E., Kazanceva M., Stom D.I., Kratasyuk V. Application of bioluminescent enzymatic method for assessment of the state of the soil //LUMINESCENCE. – 111 RIVER ST, HOBOKEN 07030-5774, NJ USA : WILEY-BLACKWELL, 2014. – Т. 29. – С. 76-77.

13. Bezrukikh, Anna; Esimbekova, Elena; Nemtseva, Elena; Kratasyuk, Valentina; Shimomura, Osamu Gelatin and starch as stabilizers of the coupled enzyme system of luminous bacteria NADH:FMN–oxidoreductase–luciferase Analytical and Bioanalytical Chemistry: Volume 406, Issue 23 (2014), Page 5743-5747.

Patents- 16

• Kratasyuk V.A., Esimbekova E.N. Bioluminescent biomodul for bioassay of toxicity of various medias and method of its preparation//Patent Russian Federation Patent Filing N 2413772, Date of Application 23.03.2009, Date of Issue 10.03.2011

• Kratasyuk V.A., Esimbekova E.N. Экспресс-способ биотестирования природных, сточных вод и водных растворов.//Patent Russian Federation Patent Filing N 2413771, Date of Application 10.04.2009, Date of Issue 10.03.2011

• Kudryasheva N.S., Kratasyuk V.A., Bioluminescent method of radioactivity monitoring //Patent Russian Federation № 2006133756, Date of Application 29.09.2006

• Kratasyuk V.A., Esimbekova E.N. Method of preparation for immobilized multicomponent reagents for bioluminescent analysis//Patent Russian Federation Patent Filing N 2252963, Date of Application 27 August 2003, Date of Issue 27 May 2005

• Sovcov S.A., Kratasyuk V.A. Determination of endotoxicosis under surgery. Owner: Institute of Biophysics (Siberian Branch of Russian Academy of Sciences) //Patent Russian Federation Patent Filing N 1714512, Date of Application 19 January 1988, Date of Issue 22 October 1991

• Kratasyuk V.A., Kovalevskiy A.N., Voevodina T.V., Shulz V.R., Nifantiev O.E. Determination of patients’ state under endotoxicosis of infectious genesis. //Patent Russian Federation Patent Filing N 1663548, Date of Application 4 February 1988, Date of Issue 15 March 1991

Grants: PI of more 40 grants.

Conferences and Exhibitions: more than 100 Russian and International (oral presentations)