Eugenia E. Bashmakova

Date of birth: February 6, 1991

Place of birth: Novomitropolka, Krasnoyarsk region, Russia

Laboratory of Bioluminescent and Environmental Technologies Institute of Biophysics

50/50 Akademgorodok 660036 Krasnoyarsk Russia

Tel: (3912) 494430

E-mail: bashmakovae@ibp.ru

EDUCATIONAL BACKGROUND

• High School, Novomitropolka, Krasnoyarsk region, Russia. High School Diploma (2008).

• Siberian Federal University, Institute of Fundamental Biology and Biotechnology. Department of Medical Biology, Krasnoyarsk, Russia. Specialist (M.S. equivalent) in Biochemistry (2013).

• Siberian Federal University, Institute of Fundamental Biology and Biotechnology, Department of Biophysics, Krasnoyarsk, Russia. Candidate of Sciences in Biology (Ph.D. equivalent), 21 September 2017. Thesis: «Detection of single nucleotide polymorphisms based on derivatives of Ca2+-regulated photoprotein obelin».

PROFESSIONAL EXPERIENCE

• 2013-2018: Laboratory of Photobiology, Institute of Biophysics SB RAS, Engineer;

• 2018: Laboratory of Photobiology, Institute of Biophysics SB RAS, Research Fellow.

• 2018-present: Laboratory of Bioluminescent and Environmental Technologies, Institute of Biophysics SB RAS, Senior Researcher

INTERNATIONAL CONFERENCES/SEMINARS ATTENDED

• 2014 X All-Russian scientific-technical conference of students, graduates and young scientists with the international participation «Youth and science», Krasnoyarsk, Russia, SibFU (Diploma for the 1st place);

• 2014 5th International conference and exhibition on Analytical & Bioanalytical Techniques, Beijing, China (Best Poster Award);

• 2014 All-Russian scientific and practical conference with international participation «Molecular Diagnostics 2014», Moscow, Russia;

• 2015 International scientific conference of young scientists biotechnologists, virologists, molecular biologists Open Bio-2015, Science town Koltsovo, Russia (Diploma for the 3st place);

• 2016 19th International Symposium on Bioluminescence and Chemiluminescence, Tsukuba, Japan;

• 2017 VIII Congress of the Russian Photobiological Society, Shepsi, Russia (Winner of the Young Scientists Competition);

• 2017 XX Conference of young scientists of Federal Research Center «Krasnoyarsk Science Center SB RAS» Krasnoyarsk, Russia (Diploma for the 3st place);

• 2018 International forum «Biotechnology: state of the art and perspectives» Moscow, Russia;

• 2018 20th International Symposium on Bioluminescence and Chemiluminescence, Nantes, France;

• 2018 III International scientific conference « Biotechnology of new materials-environment-quality of life», Krasnoyarsk, Russia (Diploma for the 1st place);

• 2019 International scientific conference of young scientists: biophysicists, biotechnologists, molecular biologists and virologists Open Bio-2019, Science town Koltsovo, Russia.

PAPERS IN PEER REVIEWD JOURNALS

1. Dunaeva E.A., Mironov K.O., Dribnohodova O.P., Subbotina, T.N., Bashmakova E.E., Olhovskii I.A., Shipulin, G.A. The quantitative testing of V617F mutation in gen JAK2 using pyrosequencing technique // Klinichescheskaya Laboratornaya Diagnostika, 2014, I.11, P. 60–63.

2. Bashmakova E.E., Krasitskaya V.V., Frank L.A. Simultaneous genotyping of four single nucleotide polymorphisms associated with risk factors of hemostasis disorders // Journal of Combinatorial Chemistry & High Throughput Screening, 2015, V. 18, I.10, P. 930–937.

3. Bashmakova E.E., Krasitskaya V.V., Bondar A.A., Kozlova A.V., Ruksha T.G., Frank L.A. A bioluminescent assay for detecting melanocortin-1 receptor (MC1R) gene polymorphisms R160W, R151C, and D294H //Molecular biology, 2015, V 49, I. 6, P. 852–857.

4. Еремина Е.Н., Башмакова Е.Е., Франк Л.А. Клиническое значение определения полиморфизмов генов у больных пигментной меланомой кожи // Сибирское медицинское обозрение, 2016, Т. 101, № 5, С. 91–92.

5. Еремина Е.Н., Зуков Р.А., Слепов Е.В., Башмакова Е.Е., Франк Л.А. Взаимосвязь однонуклеотидного полиморфизма гена рецептора меланокортина 1 и клинических особенностей течения меланомы кожи // Злокачественные опухоли, 2016, № 4-S1 (20), С. 204–205.

6. Bashmakova E.E., Krasitskaya V.V., Kudryavtsev A.N., Grigorenko V.G., Frank L.A. Hybrid minimal core streptavidin–obelin as a versatile reporter for bioluminescence-based bioassay // Photochemistry and Photobiology, 2017, V. 93, I. 2, P. 548–552.

7. Krasitskaya V.V., Burakova L.P., Komarova A.A., Bashmakova E.E., Frank L.A. Mutants of Ca2+ -regulated photoprotein obelin for site-specific conjugation // Photochemistry and Photobiology, 2017, V. 93, I. 2, P. 553–557.

8. Frank L.A., Bashmakova E.E., Krasitskaya V.V., Kudryavtsev A.N. Genetically modified coelenterazine-dependent luciferases as reporters for in vitro assay // Журнал Сибирского федерального университета. Серия: Биология, 2017, Т. 10, № 2, С. 199–210.

9. Krasitskaya V.V., Bashmakova E.E., Dobretsov K.G. Orlova N.V., Frank, L.A. The genetic aspects of occupational hearing impairment // Vestnik Otorinolaringologii, V. 82, I.5, 2017, P. 71–76.

10. Добрецов К.Г., Красицкая В.В., Башмакова Е.Е., Франк Л.А. Взаимосвязь генетических мутаций и возникновения профессиональной нейросенсорной тугоухости // Клиническая больница, 2017, № 4 (22), С. 22–25.

11. Vorobyeva M., Davydova A., Shatunova E., Vorobjev P., Tupikin A., Kabilov M., Bashmakova E., Krasitskaya V., Frank L., Venyaminova A. Novel 2 "-F-RNA aptamers specific to protein markers of glycemia - a basis for bioluminescent aptasensors // FEBS OPEN BIO, 2018, V. 1, P. 118.

12. Bashmakova E.E., Krasitskaya V.V., Bondar A.A., Eremina E.N., Slepov E.V., Zukov R.A., Frank L.A. Bioluminescent SNP genotyping technique: Development and application for detection of melanocortin 1 receptor gene polymorphisms // Talanta, 2018, V.189, P. 111–115.

13. Frank L.A., Bashmakova E.E., Goncharova N.S., Krasitskaya V.V. Bioluminescent binding microassay using aptamers as biospecific elements // J. Sib. Fed. Univ. Biol., 2019, 12(3), 244 – 252.

14. Davydova A., Vorobyeva M., Bashmakova E., Vorobjev P., Krasheninina O., Tupikin A., Kabilov M., Krasitskaya V., Frank L., Venyaminova A. Development and characterization of novel 2"-F-RNA aptamers specific to human total and glycated hemoglobins // Analytical Biochemistry, 2019,V. 570, P. 43–50.

15. Bashmakova E.E., Krasitskaya V.V., Zamay G.S., Zamay T.N., Frank L.A. Bioluminescent aptamer-based solid-phase microassay to detect lung tumor cells in plasma // Talanta, 2019. V. 199, P. 674 – 678.

16. Krasitskaya V.V., Goncharova N.S., Biriukov V.V., Bashmakova E.E., Kabilov M.R., Baykov I.K., Sokolov A.E., Frank L.A. The Ca2+-Regulated Photoprotein Obelin as a Tool for SELEX Monitoring and DNA Aptamer Affinity Evaluation // Photochem Photobiol, 2020. doi: 10.1111/php.13274

Patents

• Патент РФ 2685936 Башмакова Е.Е., Франк Л.А., Красицкая В.В. Способ выявления мишеней, ассоциированных с определенным диагнозом, в крови пациентов на основе ДНК-аптамеров. Опубликовано: 23.04.2019 Бюл. № 12

Research support in the last five years:

• Российский фонд фундаментальных исследований:

  • 16-44-242097 (2016-2018 гг), исполнитель

  • 18-38-00531 (2018-2019 гг), исполнитель.

• РНФ:

  • 14-14-01119 (2014 – 2016 гг), исполнитель.

  • 14-35-00107 (2014 – 2016 гг), исполнитель.

  • 16-14-10296 (2016 – 2018 гг), исполнитель.

• Грант Фонда содействия развития малых форм предприятия в научно-технической сфере, Программа «УМНИК-2014» (2014 – 2017 гг), руководитель.

• Грант президента РФ для молодых ученых - кандидатов наук МК-772.2020.4 (2020 – 2021 гг), руководитель.