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Name: Nadezhda S. Kudryasheva,
Titles: Professor, Dr.of Science, Physics and Mathematics,
Specialty: Biophysics
Address: Institute of Biophysics, Siberian Branch of Russian Academy of Sciences
Krasnoyarsk, 660036, Russia
Tel.: 7(391)249-42-42
Fax: 7(391)243-34-00
Education
1977-1982: Student, Chemical Department, Moscow State University, Moscow, USSR
1982-1987: Post-graduate student, Luminescence Laboratory, L.Y.Karpov Physico-Chemical Research Institute, Moscow, USSR
Degrees
1982 Degree: M.Sc., Chemistry, Moscow State University, Moscow, USSR
1991 Degree: Ph.D., Biophysics. Thesis: Upper electron-excited states in photoreduction of carbonyl compounds and in bioluminescence.
2004 Degree: Dr.Sc., Biophysics, Thesis: Mechanism of electron-excited states formation in bioluminescence.
2007 Degree: Professor
Employment:
1988 – present: Junior researcher, Researcher, Senior Researcher, Leading Researcher, Photobiology Laboratory, Institute of Biophysics, Russian Academy of Sciences, Siberian Branch, Krasnoyarsk, Russia
1992 - 2006: Lecturer, Associate Professor, Professor. Department of Physical Chemistry, Faculty of Chemistry, Krasnoyarsk State University, Krasnoyarsk, Russia.
2003 – 2006: Professor, Department of Physical Chemistry, Krasnoyarsk State University of Non-ferrous Metals and Aurum, Krasnoyarsk, Russia.
2007 – present: Professor, Siberian Federal University, Krasnoyarsk, Russia.
2007 – present: Professor, Technological University, Lesosibirsk Branch, Krasnoyarsk region, Russia.
Professional activities:
1999-2000 – Visit Researcher in Microspectroscopic Cener of Agro University, Wageningen, The Netherlands
2008-2009 – Visiting professor (Fulbright scholar), Texas Tex University, Texas, USA
2004-present –member of Scientific Council on Biophysics, Krasnoyarsk, Russia.
Main research interests:
Physical chemistry of biological objects, spectroscopy, structure of molecules, chemiluminescence, bioluminescence, primary physics-chemical processes in biological systems, bioluminescence applications in ecology: bioluminescent monitoring of detoxification processes, low-dose radiotoxicity.
PUBLICATIONS
Publications: Total number: appr. 300
Review articles in scientific journals
N. S. Kudryasheva. Bioluminescence and exogenous compounds. Physico-chemical basis for bioluminescent assay. J.Photochem.Photobiol B., 2006, V. 83, 77-86.
N.S. Kudryasheva. Nonspecific effects of exogenous compounds on bacterial bioluminescent enzymes: Fluorescence study. Current Enzyme Inhibition, 2006, V.2, N4, 363-372.
E.V. Nemtseva, N.S. Kudryasheva. The mechanism of electronic excitation in bacterial bioluminescent reaction. Uspekhi khimii, 2007, Vol. 76, N1, p.101-112. (In Russian)
A.S. Tarasova, N.S. Kudryasheva. Pollutant toxicity and detoxification by humic substances: mechanisms and quantitative assessment via luminescent biomonitoring. Environmental Science and Pollution Research. 2015, V.187, N 3:89, p.1-8. DOI: 10.1007/s10661-015-4304-1
N.S. Kudryasheva, T.V. Rozhko. Effect of low-dose ionizing radiation on luminous marine bacteria: radiation hormesis and toxicity. J. Environ. Radioact. 2015. V. 142, p.68-77. doi:10.1016/j.jenvrad.2015.01.012
Roza R. Alieva, Nadezhda S. Kudryasheva. Variability of Fluorescence Spectra of Coelenteramide-Containing Proteins as a Basis for Toxicity Monitoring. Talanta, 2017, 170, 425-431. DOI: 10.1016/j.talanta.2017.04.043
Main publications.
Kudryasheva N.S., Shigorin D.N. Carbonyl compounds photoreduced efficiency in the presence of molecular energy acceptors. Zhurn.fis.khim., 1988, T.62, N9, 2526-2528. (in Russian)
Kudrasheva N.S., Valkova G.A., Shigorin D.N., Gorelic M.V. Photochemical properties of carbonyl compounds belonging to various spectral-luminescent groups, Zhurn.fis.khim., 1989, V.63, N19, 177-183. (in Russian)
Kratasyuk V.A., Makurina V.I., Kuznetsov A.M., Kudryasheva N.S., Plotnikova N.B., Medvedeva S.E., Gritsenko I.S., Chernykh V.P. Studying of amberic acid sulpho-derivetives action on bacterial bioluminecsence. Prikladnaya biokhimiya i microbiologiya, 1991, V.27, N1, 127-133. (in Russian)
Kudrasheva N.S., Belobrov P.I., Kratasyuk V.A., Shigorin D.N. Electron excited states in bioluminescence. Dokl.AN USSR, 1991, V.321, N4. 837-841. (in Russian)
Kudrasheva N.S., Valkova G.A., Shigorin D.N. Lower triplet states orbital nature and photochemical activity of 2-phenyl-9,10-anthraquinone. Zhurn.fis.khim., 1992, V.66, N11, 2981-2985.(in Russian)
Kudryasheva N.S., Shalaeva Ye.V., Zadorozhnaya Ye.N., Kratasyuk V.A., Balayan A.E., Stom D.I. Patterns of inhibition of bacterial bioluminescence in vitro by quinones and phenols - components of sewage. Biophysics, 1994, V.39, N 3, 441-451. .(in Russian)
Kudryasheva N.S., Kratasyuk V.A., Belobrov.P.I. Bioluminescent analysis. The action of toxicants: physical-chemical regularities of the toxicants effects. Anal.Letters, 1994, V.27, N 15, 2931-2947.
Kudryashrva N.S., Zuzikova L.V., Gutyk T.V.,Kuznetsov A.M. Effects of the metallic salts on the bioluminescent systems of different complexity. Biofizika, 1996, V.41, N 6, 1264-1269. (in Russian)
Kudryasheva N.S., Kratasyuk V.A., Esimbekova E.N.,Vetrova, Kudinova I.Y. Development of the bioluminescent bioindicators for analysese of pollutions. Field Analytical Chemical Technologies, 1998, V.2, N5, 277-280.
Kratasyuk V.A., Egorova O.I., Kudryasheva N.S., L’vova L.S., Orlova H.Yu., Bytev V.O. Influence of fuzariotoxines on bacterial biluminescence in vitro. Prikladnaya biokhimiya i mikrobiologiya, 1998, V.34, N2, 207-209 (in Russian)
Kudryasheva N.S., Kudinova I.Y., Esimbekova E.N., Kratasyuk V.A., Stom D.I. Effects of quinones and phenols on the NAD(H)-dependent triple systems. Chemosphere, 1999, V.38, N4, 751-758.
Kudryasheva N.S., Zuzikova E.V., Gutnyk T.V. Mechanism of influence of metallic salts on the bioluminescent system in vitro. Biophysika, 1999, V.44, N2, 244-250. (in Russian)
N.S. Kudryasheva. Mechanisms of xenobiotics’ effects on bacterial bioluminescence, Luminescence, 1999, V.14, 199-200.
Kratasyuk V.A., Vetrova E.V., Kudryasheva N.S. Bioluminescent Water Quality Monitoring of Salt Lake Shira, Luminescence, 1999, V.14, 193-195
Kudryasheva N.S. Shilova E.V., Khendogina E.V., Kratasyuk V.A. Lake Shira, a Siberian lake: ecosystem structure and function. 3: The use of bioluminescent biotests to monitor ecological status. International Journal of Salt Lake Research, 1999, V.8, 245-251.
Kudryasheva N.S,Nemtseva E.V, Meshalkin Yu.P., Sizykh A.G.. Upper elecrton-excited states in bioluminescence: experimental indication. Luminescence, 2001, V.3, 243-246.
Kudryasheva NS, Kratasyuk VA, Esimbekova EN. Physico-chemical basis of bioluminescent bioassay, Krasnoyarsk, KrasGU Publisher, 2002, 146 p. (in Russian).
Vetrova E.V, Kratasyuk V.A, Kudryasheva N.S. Bioluminescent characteristics map of Shira lake water// Aquatic Ecology, 2002, V 36, N2-4, 309-315.
Gerasimova M.A., Kudryasheva N.S. Effects of potassium halides on bacterial bioluminescence. J.Photochem.Photobiol, 2002, V.66, N3N, 218-222.
Kudryasheva NS, Vetrova EV, Kuznetsov AM, Kratasyuk VA, Stom DI. Bioluminescent assays: effects of quinones and phenols// Ecotoxicology and Environmental Safety, 2002, V.53, N2, 221-225.
Kudryasheva N.S., Nemtseva E.V., Sizykh A.G., Kratasyuk V.A., Visser A.J.W.G. Estimation of energy of the upper electron-excited states of the bacterial bioluminescent emitter, J.Photochem.Photobiol. B, 2002, Vol.68, N2-3, 88-92.
Kudryasheva N.S., Nemtseva E.V., Visser A.J.W.G., van Hoek A. Interaction of aromatic compounds with Photobacterium leiognathi luciferase: fluorescence anisotropy study. Luminescence, 2003, V.18, N3, 156-161.
Kudryasheva N.S., Esimbekova E.N., Remmel N.N., Kratasyuk V.A., Visser A.J.W.G., van Hoek A. Effect of quinones and phenols on the triple enzymic bioluminescent system with protease. Luminescence, 2003, V.18, 224-228.
Kudryasheva N.S., Nemtseva E.V, Kirillova T.N. Exogenous compounds in studying the mechanism of electron-excited states formation in bioluminescence. Biopolymers 2004, Vol.74, N 1-2, 100-104.
Vetrova E.V., Kudryasheva N.S., Visser A.J., von Hoek A. Characteristics Of endogenous flavin fluorescence of Photobacterium leiognathi luciferase and Vibrio fischeri NAD(P)H:FMN-oxidoreductase. Luminescence, 2005, V.20, N3, 205-209.
Fedorova E.S., Kudryasheva N.S., Kuznetsov A.M., Stom D.I., Belyi A.V., Sizykh A.G. Detoxication of solutions of organic oxidants by humic substances: Bioluminescent monitoring. Doklady Biochemistry and Biophysics, 2005, V.403, N4, 300-302.
O. Tchaikovskaya, I. Sokolova, V. Svetlichnyi, E. Karetnikova, E. Kudryasheva, E. Fedorova, Luminescent analysis of photoinduced detoxication of Phenol. Zhurnal Prikladnoy Spektroskopii, 2006, Vol.73, N6, P.665-670. (In Russian)
Tatiana V. Rozhko, Nadezhda S. Kudryasheva, Alexander M. Kuznetsov, Galina A. Vydryakova, Lydia G. Bondareva and Alexander Ya. Bolsunovsky. Effect of low-level ?-radiation on bioluminescent assay systems of various complexity, Photochem. Photobiol. Sci., 2007, V.6, p.67-70.
Vetrova E.V., Kudryasheva N.S., Kratasyuk V. A. Redox compounds influence on the NAD(P)H:FMN-oxidoreductase – luciferase bioluminescent system. Photochem. Photobiol. Sci., 2007. V.6, p.35-40.
O. Tchaikovskaya, I. Sokolova, V. Svetlichnyi, E. Karetnikova, E. Fedorova, N. Kudryasheva. Fluorescent and bioluminescent analysis of sequential biological UV degradation of p-cresol in water, Luminescence, 2007, Vol.22, N1, p.29-34.
Kirillova T.N., Kudryasheva N.S. Effect of heavy atom in bioluminescent reactions// Analytical and Bioanalytical Chemistry. 2007, Vol.387, N6, p.2009-2016
Fedorova E.S., Kudryasheva N.S., Kuznetsov A.M., Mogil’naya O.A., Stom D.I. Bioluminescent monitoring of detoxi.cation processes: Activity of humic substances in quinone solutions, J. Photochem. Photobiol. B: 2007, Vol 88, N2-3. pp 131-136.
Belogurova NV, Kudryasheva NS, Alieva RR, Sizykh AG.Spectral components of bioluminescence of aequorin and obelin. J Photochem Photobiol B. 2008, Vol. 92, N2, 117-122.
Elena V Vetrova, Nadezhda S Kudryasheva, Kwan H Cheng. Effect of quinone on the fluorescence decay dynamics of endogenous flavin bound to bacterial luciferase, Biophys.Chem, 2009, Vol. 141, N 1, P.59-65.
N.V. Belogurova, N.S. Kudryasheva, R.R. Alieva. Activity of upper electron-excited states in bioluminescence of coelenterates. J.Mol. Str., 2009 Vol. 924–926 p.148–152.
Kudryasheva NS, Kirillova TV, Vasyunkina EV. Classification of effects of Exogenous compounds on Bioluminescent Assay Systems. Luminescence, 2010, V.25, N2, P.151-152.
M. Alexandrova, T. Rozhko, G.Badun, L.Bondareva, G.Vydryakova, A.Bolsunovsky, N.Kudryasheva. Effect of Tritium on Growth and Luminescence of P.Phosphoreum. Luminescence, 2010, V.25, N2, P.125-126.
M. Alexandrova, T. Rozhko, N.Kudryasheva, G.Vydryakova, A.Bolsunovsky. Effect of Americium-241 on luminous bacteria. Luminescence, 2010, V.25, N3, P.241-242.
N. Belogurova, N. Kudryasheva. Discharged Photoprotein Obelin: Fluorescence Peculiarities. J.Photochem.Photobiol.B, 2010, V.101, N1, P.103–108.
M. Alexandrova, T. Rozhko, G. Vydryakova, N. Kudryasheva. Effect of americium-241 on luminous bacteria. Role of peroxides. Journal of Environmental Radioactivity, 2011, V.102, P. 407-411.
T.V. Rozhko, L.G. Bondareva, O.A. Mogilnaya, G.A. Vydryakova, A.Ya. Bolsunovsky, D.I. Stom, N.S. Kudryasheva. Detoxification of Am-241 Solutions by Humic Substances: Bioluminescent Monitoring. Anal.&Bioanal.Chem. 2011, V.400, N2, p.329–334.
Anna S. Tarasova, Devard I. Stom, Nadezhda S. Kudryasheva. Bioluminescent toxicity monitoring of oxidizer solutions. Effect of humic substances. Environmental Toxicology and Chemistry, 2011, Vol.30, N 5, P.1013-7.
T.N. Kirillova, M.A. Gerasimova, E.V. Nemtseva, N.S. Kudryasheva. Effect of halogenated fluorescent compounds on bioluminescent reactions Anal.&Bioanal.Chem. 2011, V. 400, N2, p.343-51
A.S. Tarasova, S.L. Kislan, E.S. Fedorova, A.M. Kuznetsov, O.A. Mogilnaya, D.I. Stom, N.S. Kudryasheva. Bioluminescence as a tool for studying detoxification processes in metal salt solutions involving humic substances. Journal of Photochemistry and Photobiology B, 2012, V. 117, N5. P. 164–170.
Kamnev A.A., Tugarova A.V., Selivanova M.A , Tarantilis P.A., Polissiou M.G., Kudryasheva N.S. Effects of americium-241 and humic substances on Photobacterium phosphoreum: bioluminescence and diffuse reflectance FTIR spectroscopic studies. Spectrochim. Acta A: Mol. Biomol. Spectrosc. 2013, Vol.100, P.271-275.
Roza R. Alieva, Nadezhda V. Belogurova, Alena S. Petrova, Nadezhda S. Kudryasheva. Fluorescence properties of Ca2+-independent discharged obelin and its application prospect. Anal.&Bioanal.Chem. 2013. V. 405, N 10, P. 3351-3358.
M.A. Selivanova, O.A. Mogilnaya, G.A. Badun, G.A. Vydryakova, A.M. Kuznetsov, N.S. Kudryasheva. Effect of tritium on luminous marine bacteria and enzyme reactions. Journal of Environmental Radioactivity, 2013, 2013, V.130. - P. 19-25.
Roza R. Alieva, Nadezhda V. Belogurova, Alena S. Petrova, Nadezhda S. Kudryasheva. Effects of alcohols on fluorescence intensity and color of a discharged-obelin-based biomarker. Anal&Bioanal. Chem, 2014, 406 (12), 2965-2974.
M. A. Selivanova, T. V. Rozhko, A. N. Devyatlovskaya, N. S. Kudryasheva. Comparison of chronic low-dose effects of alpha- and beta-emitting radionuclides on marine bacteria. 2014, Cent Eur J Biol, V. 9 (10), pp 951-959.
A. S. Tarasova, D. I. Stom, N. S. Kudryasheva. Antioxidant activity of humic substances via bioluminescent monitoring in vitro. 2015, Environmental Monitoring and Assessment, V.187, N 3:89, p.1-8. DOI: 10.1007/s10661-015-4304-1
T.V. Rozhko., G.A. Badun, I.A. Razzhivina, O.A. Guseynov, V.E. Guseynova, N.S. Kudryasheva, On the mechanism of biological activation by tritium J. Environ. Radioact. 2016. V.157. p.131-135. DOI: 10.1016/j.jenvrad.2016.03.017
AS Petrova, RR Alieva, NV Belogurova, LS Tirranen, NS KudryashevaVariation of Spectral Characteristics of Coelenteramide-Containing Fluorescent Protein from Obelia Longissima Exposed to Dimethyl Sulfoxide Russian Physics Journal 2016, 59 (4), 562-567; doi:10.1007/s11182-016-0806-8
RR Alieva, FN Tomilin, AA Kuzubov, SG Ovchinnikov, NS KudryashevaUltraviolet fluorescence of coelenteramide and coelenteramide-containing fluorescent proteins. Experimental and theoretical study. Journal of Photochemistry and Photobiology B: Biology 2016, 162, 318-323
Kudryasheva, N.S.; Petrova, A.S.; Dementyev, D.V.; Bondar, A.A. Exposure of luminous marine bacteria to low-dose gamma-radiation. Journal of Environ Radioactivity, 2017, V.169-170, p. 64-69. DOI: 10.1016/j.jenvrad.2017.01.002
Nadezhda S. Kudryasheva, Ekaterina S. Kovel, Anna S. Sachkova, Anna A. Vorobeva, Viktoriya G. Isakova, Grigoriy N. Churilov. Bioluminescent Enzymatic Assay as a Tool for Studying Antioxidant Activity and Toxicity of Bioactive Compounds. Photochemistry and Photobiology. 2017. 93 (2), 536-540. DOI: 10.1111/php.12639
A.S. Sachkova , E.S. Kovel, G.N. Churilov, O.A. Guseynov, A.A. Bondar, I. A. Dubinina, A.A. N.S. Kudryasheva. On mechanism of antioxidant effect of fullerenols. Biochemistry and Biophysic Reports, Biochemistry and Biophysics Reports. 2017, 9, 1–8. DOI:10.1016/j.bbrep.2016.10.011
Petrova A.S., Lukonina A.A., Badun G.A., Kudryasheva N.S. Fluorescent Coelenteramide-Containing Protein as a Color Bioindicator for Low-Dose Radiation Effects. Anal&Bioanal. Chem, 2017. V. 409, N18, pp 4377–4381. DOI: 10.1007/s00216-017-0404-9
T.V. Rozhko, O.A. Guseynov, А.А. Bondar, V.E. Guseynova, A.N. Devyatlovskaya, N.S. Kudryasheva. Is bacterial luminescence response to low-dose radiation associated with mutagenicity? Journal of Environ Radioactivity, 2017. 177, 261-265
Alena S. Petrova, Anna A. Lukonina, Dmitry V. Dementyev, Alexander Ya. Bolsunovsky, Anatoliy V. Popov, Nadezhda S. Kudryasheva. Protein-based fluorescent bioassay for lowdose gamma radiation exposures. Analytical and Bioanalytical Chemistry. 2018.410, N 26 , P. 6837-6844. DOI 10.1007/s00216-018-1282-5
Anna S. Sachkova, Ekaterina S. Kovel, Grigoriy N. Churilov, Devard I. Stom, Nadezhda S. Kudryasheva. Biological activity of carbonic nano-structures. Comparison via enzymatic bioassay. Journal of Soils and Sediments. 2018 DOI: 10.1007/s11368-018-2134-9
Text books: 19 text-books and work-books
Patents:
Patent of Russian Federation N 2006133756. Bioluminescent method for monitoring of radiotoxicity in solutions. Priority from 21.09.2006.
Patent: Bioluminescent method for monitoring the radiotoxicity of solutions. International Application No PCT/ Ru 2007/000239, International filing date 14 May 2007. Priority Date 2006133756 21.09.2006 RU
Patent of Russian Federation N 2376380, 20.12.2009. Kudryasheva NS, Fedorova EC, Bioluminescent method for determination of antioxidant activity of humic substances. Priority from 29.06.2007.
Patent of Russian Federation N2411000, 10.02.2011. Kudryasheva NS, Belogurova N. V., Fluorescent method for determination of Calcium concentration. Priority from 05.08.2009.
MEMBERSHIP IN SCIENTIFIC SOCIETIES
Society of Environmental Toxicology and Chemistry (SETAC), Coordinator of Siberian sub-Branch, Russian Language Branch, European division of SEARC
International Humic Substances Society
Russian Society of Photobioligists
Tutorials
Kudryasheva NS, Kratasyuk VA, Esimbekova EN. Physico-chemical basis for bioluminecent analysis. 2002, Krasnoyarsk, Krasnoyarsk State Univ., 146 p.
Kudryasheva NS, Kratasyuk VA, Esimbekova EN. Physico-chemical basis for bioluminecent analysis. Titorial. 2002, Krasnoyarsk, Krasnoyarsk State Univ., Electronic library of system of aducational portals of Ministry of education and Science RF. N 08-06/2491. http: window.edu.ru/window/library:
Kudryasheva NS, Grouns for Physical Chemistry. Tutorial, GOU VPO “State Univ. of nonferrous metals and gold. Krasnoyarsk, 2006. 120 p.
Kudryasheva NS, Bondareva LG. Physical Chemistry. Tutoriai for bachelors, Moscow, You-write Publishing House, Moscow, 2012, 345 P
Kudryasheva NS, Bondareva LG. Physical oid Chemistry. Tutoriai for bachelors, Moscow, You-write Publishing House, Moscow, 2014, ser 60. Applied course (1-st edition)
Kudryasheva NS, Bondareva LG. Physical and Colloid Chemistry. Tutoriai and Practicum, Moscow, You-write Publishing House, Moscow, 2015, ser 68. (2-nd edition)
Supervision of PhD degrees
2002. Nemtseva E.V., Biophysics (Physics and Mathematics)
2002. Vetrova E.V., Biophysics (Biology)
2008. Rozhko T.V., Biophysics (Biology)
2010. Belogurova N.V., Biophysics (Physics and Mathematics)
2011. Fedorova E.V., Biophysics (Biology)
2012. Tarasova A.S., Biophysics (Biology)
2013. Selivanova M.A., Biophysics (Biology)
2017 Alieva R.R. Biophysics (Biology)
Гранты:
РФФИ: №18-44-240004 р_а. Роль активных форм кислорода в клеточных процессах при низкодозовых радиационных нагрузках на примере морских бактерий и клеток периферической крови сельскохозяйственных животных. (Руководитель)
РФФИ: №18-44-242002 р_мк. Флуоресцентный белок как принципиально новый биосенсор для мониторинга токсичности (Руководитель)
Программа РАН Подпрограмма: III. №32. Нанобиотехнологии. 1.Нанодетекция и нанодиагностика. Проект: «Токсические и антиоксидантные свойства углеродных наноструктур – фуллеренолов.» 2018-2020 (Руководитель)
РФФИ: №15-43-04377 р_сибирь_а. Биолюминесцентные системы как инструмент оценки антиоксидантной активности физиологически-активных веществ. (Руководитель)
РФФИ: №15-03-06786 А. Теоретическое и экспериментальное исследование механизма взаимодействия водорастворимых фуллеренолов с активными формами кислорода в растворах модельных окислителе (Исполнитель)
РНФ: №14-14-00076 Оценка степени радиочувствительности разных видов организмов при биотестировании радиоактивного загрязнения экосистем на примере реки Енисей (Исполнитель)
РФФИ: №13-04-98072 р_сибирь_а. Детоксикация растворов солей металлов гуминовыми веществами. Биолюминесцентный мониторинг. (Руководитель)
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