Valentin N. Petushkov, Ph.D

CURRICULUM VITAE

Academic Education:

1969–1974 – Faculty of Physics, Krasnoyarsk State University, Krasnoyarsk, Russia, USSR

Scientific Degrees:

1986, Ph.D. (Biophysics) "Bienzymatic system of NADH:FMN-oxidoreductase-luciferase from luminescent bacteria"

Academic Career:

• 1980–present – researcher, post-doc, senior researcher, Institute of Biophysics, Siberian Branch of Russian Academy of Sciences, Krasnoyarsk

• 1977–1979 – Junior Researcher, Scientist, Photobiology laboratory, L.V.Kirensky Institute of

• Physics (USSR Academy of Sciences, Siberian Branch), Krasnoyarsk

Current position:

Senior Researcher, Institute of Biophysics, Siberian Branch of Russian Academy of Sciences, Krasnoyarsk

Temporal Positions

• 1993-1995 – Project “Antenna proteins”, Postdoctoral Associate, Department of Biochemistry & Molecular Biology, University of Georgia, Athens, Georgia, USA

• 1996-1997 – Project “Antenna proteins”, Postdoctoral Associate, Wageningen Agriculture University, Netherlands

• 1998, 1999, 2001 – visiting scientist in research group in the Center of Genomiuc Reserch at the Karolinska Institute in Stockholm, Sweden

Research Interests: chemiluminescence and bioluminescence, luminescent organisms, luciferases and luciferins, enzyme kinetics, structure of molecules, physics-chemical processes in luminescent systems, bioluminescent analysis

Research Activities:

• 1977–1992 – Investigation of biophysical and biochemical mechanisms of bacterial bioluminescence

• 1992–2001 – Investigation of fluorescent resonance energy transfer from bacterial luciferase to antenna proteins

• Since 2001 – Investigation of mechanism of new types of luminescent systems of soil enchytraeids (Annelida: Clitellata: Oligochaeta: Enchytraeidae)

Grant Support:

Active:

• 2014-2018 – Russian Science Foundation (grant 14-50-00131) "Белки и пептиды в постгеномную эру. Структурно-функциональные исследования для решения фундаментальных задач и направленного конструирования инновационных лекарственных средств." Investigator.

• 2015-2018 – Russian Science Foundation (grant 16-14-00052) "Расшифровка молекулярного механизма биолюминесценции высших грибов и разработка методических основ ее использования с целью создания новой патентно чистой линии аналитических методов для биотехнологии и медицины" Investigator.

Completed:

• 2015-2017 – Russian Fund of Fundamental Investigation (RFFI 15-04-04414 A) “Isolation, studying of properties and structure elucidation of a substrate of luminescent reaction of luminous fungus Neonothopanus nambi.” Investigator.

• 2015-2017 – Russian Fund of Fundamental Investigation (RFFI 15-04-02695 A) “The novel сalcium-dependent luminescent system of soil enchytraeid Henlea sp.” Principal investigator.

Patents:

• Patent RF 2596398. Method and agents for detecting luciferase activity. I.V.Yampol"skiy, V.N.Petushkov, K.V.Purtov, N.S.Rodionova, M.S.Baranov. Published on September 10, 2016.

• Patent US 20180163248. Method and agents for detecting luciferase activity. I.V.Yampol"skiy, V.N.Petushkov, K.V.Purtov, N.S.Rodionova, M.S.Baranov. Published on June 14, 2018.

Publications in the last five years:

1. V.N.Petushkov, A.S.Tsarkova, M.A.Dubinnyi, N.S.Rodionova, S.M.Marques, J.C.Esteves da Silva, O.Shimomura, I.V.Yampolsky. CompX, a luciferin-related tyrosine derivative from the bioluminescent earthworm Fridericia heliota. Structure elucidation and total synthesis. Tetrahedron Letters. (2014) 55: 460-462.

2. V.N.Petushkov, A.S.Dubinnyi, N.S.Rodionova, K.D.Nadezhdin, S.M.Marques, J.C.Esteves da Silva, O.Shimomura, I.V.Yampolsky. AsLn2, a luciferin-related modified tripeptide from the bioluminescent earthworm Fridericia heliota. Tetrahedron Letters. (2014) 55: 463-465.

3. V.N.Petushkov, M.A.Dubinnyi, A.S.Tsarkova, N.S.Rodionova, M.S.Baranov, V.S.Kublitski, O.Shimomura, I.V.Yampolsky. A Novel Type of Luciferin from the Siberian Luminous Earthworm Fridericia heliota: Structure Elucidation by Spectral Studies and Total Synthesis. Angew. Chem. Int. Ed. Engl., (2014) 53(22): 5566–5568.

4. A.S.Tsarkova, M.A.Dubinnyi, M.S.Baranov, V.N.Petushkov, N.S.Rodionova, M.B.Zagudaylova, I.V.Yampolsky. Total synthesis of AsLn2 – a luciferin analogue from the Siberian bioluminescent earthworm Fridericia heliota. Mendeleev Commun. (2015) 25(2): 99-100.

5. M.A. Dubinnyi, A.S.Tsarkova, V.N.Petushkov, Z.M.Kaskova, N.S.Rodionova, S. I.Kovalchuk, R.H.Ziganshin, M.S.Baranov, K.S.Mineev, I.V.Yampolsky. Novel Peptide Chemistry in Terrestrial Animals: Natural Luciferin Analogues from the Bioluminescent Earthworm Fridericia heliota. Chemistry - A European Journal, (2015) 21(10): 3942-3947.

6. K.V.Purtov, V.N.Petushkov, M.S.Baranov, K.S.Mineev, N.S.Rodionova, Z.M.Kaskova, A.S.Tsarkova, A.I.Petunin, V.S.Bondar, E.K.Rodicheva, S.E.Medvedeva, Y.Oba, Y.Oba, A.S.Arseniev, S.Lukyanov, J.I.Gitelson, I.V.Yampolsky. The chemical basis of fungal bioluminescence. Angew. Chem. Int. Ed. Engl., (2015) 54(28): 8124–8128.

7. M.A.Dubinnyi, Z.M.Kaskova, N.S.Rodionova, M.S.Baranov, A.Yu.Gorokhovatsky, A.Kotlobai, K.M.Solntsev, A.S.Tsarkova, V.N.Petushkov, I.V.Yampolsky. Novel Mechanism of Bioluminescence: Oxidative Decarboxylation of a Moiety Adjacent to the Light Emitter of Fridericia Luciferin. Angew. Chem. Int. Ed. Engl., (2015) 54(24): 7065-7067.

8. Z.M.Kaskova, F.A.Dorr, V.N.Petushkov, K.V.Purtov, A.S.Tsarkova, N.S.Rodionova, K.S.Mineev, E.B.Guglya, A.Kotlobay, N.S. Baleeva, M.S.Baranov, A.S. Arseniev, J.I.Gitelson, S.Lukyanov, Y.Suzuki, S.Kanie, E.Pinto, P.Di Mascio, H.E.Waldenmaier, T.A.Pereira, R.P.Carvalho, A.G.Oliveira, Y.Oba, E.L.Bastos, C.V.Stevani, I.V.Yampolsky. Mechanism and Color Modulation of Fungal Bioluminescence. Science Advances. (2017) 3(4) e1602847 Published online. http://advances.sciencemag.org/content/3/4/e1602847.full

9. K.V.Purtov, V.N.Petushkov, N.S.Rodionova, J.I.Gitelson. Why Does the Bioluminescent Fungus Armillaria mellea Have Luminous Mycelium but Nonluminous Fruiting Body? Doklady Biochemistry and Biophysics. (2017) 474(1): 217–219.

10. K.V.Purtov, Z.M.Osipova, V.N.Petushkov, N.S.Rodionova, A.S.Tsarkova, A.A.Kotlobay, T.V.Chepurnykh, A.Yu.Gorokhovatsky, I.V.Yampolsky, J.I.Gitelson. Structure of fungal oxyluciferin, the product of the bioluminescence reaction. Doklady Biochemistry and Biophysics. (2017) 477(1): 360-363.

11. E.Rota, S.Martinsson, C.Erseus, V.N. Petushkov, N.S.Rodionova, P.Omodeo. Green light to an integrative view of Microscolex phosphoreus (Duges, 1837) (Annelida: Clitellata: Acanthodrilidae). ZOOTAXA. (2018) 4496(1): 175-189.

12. K.V.Purtov, A.Yu.Gorokhovatsky, A.A.Kotlobay, Z.M.Osipova, V.N.Petushkov, N.S.Rodionova, A.S.Tsarkova, T.V.Chepurnykh, I.V.Yampolsky, J.I.Gitelson. Isolation and Purification of Fungal Luciferase from Neonothopanus nimbi. Doklady Biochemistry and Biophysics. (2018) 480(1): 177-180.

13. V.N.Petushkov, N.S.Rodionova. Low-Molecular-Weight Components of Luminescent Reaction of the Siberian Enchytraeid Henlea sp. Doklady Biochemistry and Biophysics. (2018) 481: 212–216.

14. A.Kotlobay, K.S.Sarkisyan, Yu.A.Mokrushina, M.Marcet-Houben, E.O.Serebrovskaya, N.M.Markina, L.Gonzalez Somermeyer, A.Y.Gorokhovatsky, A.Vvedensky, K.V.Purtov, V.N.Petushkov, N.S.Rodionova, T.V.Chepurnykh, L.I.Fakhranurova, E.B.Guglya, R.Ziganshin, A.S.Tsarkova, Z.M.Kaskova, V.Shender, M.Abakumov, T.Abakumova, I.S.Povolotskaya, F.M.Eroshkin, A.G.Zaraisky, A.S.Mishin, S.V.Dolgov, T.Yu.Mitiouchkina, E.P.Kopantzev, H.Waldenmaier, A.G.Oliveira, Y.Oba, E.Barsova, E.A.Bogdanova, T.Gabaldon, C.V.Stevani, S.Lukyanov, I.V.Smirnov, J.I.Gitelson, F.A.Kondrashov, I.V.Yampolsky. A genetically encodable bioluminescent system from fungi. PNAS (2018) 115(49):