MARIA Yu. TRUSOVA

Laboratory of Experimental Hydroecology

Institute of Biophysics SB RAS

Akademgorodok, 660036, Krasnoyarsk, Russia

Tel: +7-903-924-84-43

E-mail: mtrusova@ibp.krasn.ru

Educational Background

• 1998, June Higher education degree (Biochemistry), Biological faculty of the Krasnoyarsk State University, Krasnoyarsk, Russia. Thesis: Effect of different factors on the process of extracellular degradation of poly-b-hydroxybutyrate granules extracted from Alcaligenes eutrophus.

• 2000 - 2003 Ph.D. student, Institute of Biophysics, Russian Acad. Sci., Siberian Branch, Krasnoyarsk, Russia.

• 2004, May Candidate of science (Ph.D.) in biophysics (03.00.02) and hydrobiology (03.00.18), thesis “Identification of species composition and study of seasonal dynamics of bacterioplankton in small eutrophic reservoirs using molecular genetics approach”

Professional Experience

• 1998 - 2000 Laboratory assistant in the Laboratory of Experimental Hydroecology, Institute of Biophysics SB RAS, Krasnoyarsk, Russia.

• 2000 - 2003 Junior research scientist, Lab. of Experimental Hydroecology, IBP SB RAS.

• 2003 - 2004 Research scientist, Lab. of Experimental Hydroecology, IBP SB RAS.

• 2004 - present Senior research scientist, Lab. of Experimental Hydroecology, IBP SB RAS.

Research experience

• Methods: molecular biology techniques (DNA & RNA extraction and clean-up, gel chromatography, PCR/qPCR, DNA cloning and sequencing, denaturing gradient gel electrophoresis), sequence data analysis, light microscopy, general microbiological techniques.

Research interests

• Phylogenetic diversity of aquatic and soil bacteria, microbial ecology, biophysics of aquatic ecosystems, biopolymers degradation

Research projects supervised:

• 2002 - 2006 Grants of Krasnoyarsk Regional Scientific Foundation for young researchers

• 2006 - 2008 U.S. Civilian Research & Development Foundation (CRDF) and RF Ministry of Education and Science, BRHE Program, grant Y3-B-02-12

• 2007 - 2008 Grant of the President of Russian Federation to young candidates of science MK-1167.2007.4

• 2009 – 2011 Russian Foundation for Basic Research, grant 09-04-01748

International conferences/seminars attended:

• 2003 1st FEMS Congress, Ljubljana, Slovenia

• 2010 31st SIL Congress, Cape Town, South Africa

• 2011 Russia-Taiwan Mini-Seminar within the framework of Taiwan-Russia Joint Research Project No. 149, National Taiwan University, Taipei, Taiwan

Papers in peer-reviewed journals:

1. Trusova M.Yu., Kolmakova O.V., Gladyshev M.I. Seasonal features of consumption of lysine by uncultivated bacterial plankton of eutrophic water reservoir. (2012) Contemporary Problems of Ecology, 5 (4):391–398. (Translated from Russian)

2. Kolmakova O.V., Trusova M.Yu. (2011) Uptake of amino acids by uncultivated bacterioplankton of a eutrophic water reservoir. Contemporary Problems of Ecology, 4 (1):8–14. (Translated from Russian)

3. Rogozin D.Y., Trusova M.Y., Khromechek E.B., Degermendzhy A.G. (2010) Microbial community of the chemocline of the meromictic Lake Shunet (Khakassia, Russia) during summer stratification. Microbiology. 79 (2):253-261. (Translated from Russian)

4. Grodnitskaya I.D., Trusova M.Y. (2009) Microbial communities and transformation of carbon compounds in bog soils of the taiga zone (Tomsk oblast). Eurasian Soil Science. 42(9):1021–1028. (Translated from Russian)

5. Volova T.G., Gladyshev M.I., Trusova M.Y., Zhila N.O. (2007) Degradation of polyhydroxyalkanoates in eutrophic reservoir. Polymer Degradation and Stability. 92(4):580-586.

6. Volova T.G., Trusova M.Y., Kalacheva G.S., Kozhevnicov I.V. (2006) Physiological–biochemical properties and the ability to synthesize polyhydroxyalkanoates of the glucose-utilizing strain of the hydrogen bacterium Ralstonia eutropha B8562. Applied Microbiology and Biotechnology. 73: 429-433.

7. Volova T.G., Gladyshev M.I., Trusova M.Yu., Zhila N.O. (2006) Degradation of polyhydroxyalkanoates and the composition of microbial destructors under natural conditions. Microbiology. 75:593-598. (Translated from Russian)

8. Trusova M.Yu., Gladyshev M.I. (2006) Experimental determination of organic substrates utilized by noncultivable bacterioplankton of a eutrophic reservoir. Doklady Biological Sciences. 409:302-304. (Translated from Russian)

9. Gladyshev M.I., Chuprov S.M., Kolmakov V.I., Dubovskaya O.P., Kravchuk Ye.S., Ivanova Ye.A., Trusova M.Yu., Sushchik N.N., Kalacheva G.S., Gubanov V.G., Prokopkin I.G., Zuyev I.V., Makhutova O.N. (2006) "Top-down" biomanipulation in a small Siberian reservoir without daphnids. Siberian Journal of Ecology. 1: 55-64. (in Russian)

10. Trusova M.Yu., Gladyshev M.I. (2005) Species composition of winter bacterioplankton in blooming and nonblooming reservoirs as determined by 16S rRNA sequences. Doklady Biological Sciences. 405:443-445. (Translated from Russian)

11. Volova T.G., Kozhevnokov I.V., Dolgopolova Yu.B., Trusova M.Yu., Kalacheva G.S., Aref’eva Yu.V. (2005) Physiological and biochemical characteristics and capacity for polyhydroxyalkanoates synthesis in a glucose-utilizing strain of hydrogen-oxidizing bacteria, Ralstonia eutropha B8562. Microbiology. 74:684-689. (Translated from Russian)

12. Volova T.G., Gladyshev M.I., Trusova M.Yu., Zhila N.O., Kartushinskaya M.V. (2004) Degradation of bioplastics in natural environment. Doklady Biological Sciences.397:330-332. (Translated from Russian)

13. Gladyshev M.I., Kravchuk E.S., Kolmakov V.I., Trusova M.Yu., Ivanova E.A., Gribovskaya I.V., Suschik N.N. (2004) A comparative study of the effect of chemical composition of water and bottom sediments on blue-green algae development in two small waterbodies. Rus. J. Biol. Inland Waters. 2:47-52. (in Russian)

14. Muchkina E. Ya., Trusova M.Yu., Gladyshev M.I., Kolmakov V.I., Dubovskaya O.P., Suschik N.N. (2003) Bacterioplankton of the suburban recreation pond and its relationships with hydrobiological, hydrochemical, and epidemiological indexes. Contemporary Problems of Ecology. 3:257-266. (in Russian)

15. Trusova M.Yu., Gladyshev M.I. (2002) Phylogenetic diversity of winter bacterioplankton of eutrophic Siberian reservoirs as revealed by 16S rRNA gene sequences. Microbial Ecology. 44 (3):252-259.

16. Sushchik N.N., Gladyshev M.I., Kalacheva G.S., Dubovskaya O.P., Kravchuk Ye.S., Ivanova Ye.A., Trusova M.Yu. (2002) The seasonal dynamics of zooplankton and contents of essential fatty acids in seston of a small pond. Rus. J. Biol. Inland Waters. 2:60-68. (in Russian)

17. Gladyshev M.I., Kravchuk Ye.S., Kolmakov V.I., Ivanova Ye.A., Trusova M.Yu., Sushchik N.N., Gribovskaya I.V. (2002) Experimental study of postwintering germination of akinetes and dormant cells of Cyanoprokaryotes from bottom sediments of blooming and nonblooming ponds. Doklady Biological Sciences.383:131-132. (Translated from Russian)

18. Trusova M.Yu., Gladyshev M.I. (2002) Species composition of winter bacterioplankton in two Siberian ponds determined by the 16S rRNA sequence analysis. Doklady Biological Sciences. 382:51-54. (Translated from Russian)

19. Gladyshev M.I., Kolmakov V.I., Kravchuk Ye.S., Kalacheva G.S., Kolmakova A.A., Gribovskaya I.V., Moskvicheva A.V., Trusova M.Yu., Zhilenkov M.D., Makhutova O.N. (2001) Germination of cyanobacterial akinetes from bottom deposits in water from blooming and nonblooming ponds under experimental conditions. Doklady Biological Sciences. 378:236-239. (Translated from Russian)

20. Gladyshev M.I., Kolmakov V.I., Kravchuk Ye.S., Ivanova Ye.A., Trusova M.Yu., Gribovskaya I.V., Zhilenkov M.D. (2000) Growth and survival of cyanobacteria in water from blooming and nonblooming ponds under experimental conditions. Doklady Biological Sciences. 375:607–609. (Translated from Russian)

21. Volova T.G., Kalacheva G.S., Trusova M.Yu., Tyul’kova N.A., Puzyr’ A.P. (1999) Intracellular degradation of poly-b-hydroxybutyrate in the hydrogen-oxidizing bacterium Alcaligenes eutrophus. Microbiology. 68:52-58. (Translated from Russian)

22. Kolmakova O.V., Gladyshev M.I., Rozanov A.S., Peltek S.E., Trusova M.Y. (2014) Spatial biodiversity of bacteria along the largest Arctic river determined by next-generation sequencing. FEMS Microbiology Ecology. 89(2): 442-450.

Membership in scientific societies:

• Russian Hydrobiological Society under RAS(from 2001)

Students supervised who earned graduate degrees:

• 2011 Kolmakova O.V., Siberian Federal University, Higher education degree (Biochemistry)